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| 產(chǎn)地 | 美國(guó) |
| 品牌 | BD |
| 貨號(hào) | 562574 |
| 用途 | 轉(zhuǎn)錄因子緩沖液 |
| 包裝規(guī)格 | / |
| 純度 | /% |
| CAS編號(hào) | |
| 別名 | 轉(zhuǎn)錄因子緩沖液 |
| 是否進(jìn)口 |
The BD Pharmingen™ Transcription Factor Buffer Set is optimized for fixing and permeabilizing cells prior to immunofluorescent staining and flow cytometric analysis of cells that express specific intracytoplasmic and intranuclear proteins. The BD Pharmingen™ Transcription Factor Buffer Set was designed to improve ease-of-use and minimize processing time, to reduce nonspecific staining, to increase the resolution of positively stained cells and to significantly reduce cell loss during fixation, permeabilization and staining procedures. Flow cytometric detection of the many proteins known to be expressed within various intracellular compartments, especially transcription factors, is improved with BD Pharmingen™ Transcription Factor Buffer Set use. This buffer system has been found useful for fixing and permeabilizing a variety of cell types from diverse human and mouse tissues. The buffer system is flexible in supporting multiwell-plate high-throughput and bulk sample analyses and applications that require overnight sample storage. The buffer system has minimal impact on the light-scatter and autofluorescence characteristics of processed cells resulting in characteristics similar to those observed for freshly prepared, highly viable primary cells. In many cases the buffer system was found to be compatible with the immunofluorescent staining of cell-surface antigens both before and after cellular fixation and permeabilization. The buffer system is also compatible with many tandem fluorochromes.
562574-BD Pharmingen™轉(zhuǎn)錄因子緩沖液套裝 現(xiàn)貨
562574-BD Pharmingen™轉(zhuǎn)錄因子緩沖液套裝 現(xiàn)貨
562574-BD Pharmingen™轉(zhuǎn)錄因子緩沖液套裝 現(xiàn)貨
BD Pharmingen™轉(zhuǎn)錄因子緩沖液套裝優(yōu)化用于在免疫熒光染色和表達(dá)特定胞質(zhì)內(nèi)和核內(nèi)蛋白的細(xì)胞的流式細(xì)胞術(shù)分析之前固定和透化細(xì)胞。
產(chǎn)品介紹：
細(xì)胞內(nèi)染色方案
1.固定：細(xì)胞表面染色程序完成后，吸出殘留的染色緩沖液并通過短暫渦旋松開細(xì)胞沉淀。向每個(gè)管中加入1mL新鮮制備的1x Fix / Perm緩沖液工作溶液，并通過渦旋振蕩約3秒重懸細(xì)胞沉淀。將樣品在2-8°C孵育40-50分鐘，避光。
2洗滌：將1ml 1x Perm /洗滌緩沖液直接加入懸浮在1x Fix / Perm緩沖液中的固定和透化細(xì)胞中。離心沉淀細(xì)胞。（注意：Fix / Perm后的所有離心步驟均為350 g ，在2-8°C下進(jìn)行6分鐘）。傾倒或吸出上清液。
3.洗滌：向沉淀的細(xì)胞中加入2ml 1x Perm /洗滌緩沖液，然后離心。傾析或吸出洗滌緩沖液。
4.細(xì)胞內(nèi)染色：向細(xì)胞樣品中加入80-100μl的1x Perm / Wash Buffer和對(duì)細(xì)胞內(nèi)蛋白質(zhì)特異的熒光抗體（如FoxP3，T-bet和/或IL-17A）和非特異性對(duì)照染色（例如，匹配熒光Ig同種型對(duì)照）到每個(gè)管。渦旋管或支架10秒鐘，在2-8°C溫育40-50分鐘，避光。
5洗滌：在洗滌之前簡(jiǎn)單地渦旋樣品。用2ml 1x Perm /洗滌緩沖液洗滌細(xì)胞。離心細(xì)胞。傾倒或吸出洗滌緩沖液。
6.洗滌：用2ml 1x Perm / Wash洗滌細(xì)胞。離心細(xì)胞。傾析或吸出洗滌緩沖液。
7.用于流式細(xì)胞術(shù)的樣品制備：將細(xì)胞沉淀重懸于350μl流式細(xì)胞術(shù)染色緩沖液中。使用流式細(xì)胞儀分析細(xì)胞并獲取數(shù)據(jù)。
562574-BD Pharmingen™轉(zhuǎn)錄因子緩沖液套裝 現(xiàn)貨
562574-BD Pharmingen™轉(zhuǎn)錄因子緩沖液套裝 現(xiàn)貨
562574-BD Pharmingen™轉(zhuǎn)錄因子緩沖液套裝 現(xiàn)貨
