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貨號： 1012-050

GMP Recombinant Human Granulocyte Macrophage-Colony Stimulating Factor (rh GM-CSF)重組人粒細(xì)胞刺激因子產(chǎn)品信息：

Data Sheet: CellGenix™

GMP Recombinant Human Granulocyte Macrophage-Colony Stimulating Factor (rh GM-CSF)

Order No.: 1012-050

Source

A DNA sequence encoding the mature GM-CSF protein (Cantrell,M. et al., 1985,PNAS 82:6250-6254; Lee, F. et al., 1985, PNAS 82:4360-4364; and Wong. et al.,1985, Science 228:810-815) was expressed in E. coli

Molecular mass

The methionyl form of the E. coli expressed mature human GM-CSF contains 128 amino acid residues and has a molecular mass of approximay 14.5 kDa

Identity

Confirmed by DNA-sequencing of the expression plasmid in end of production (EOP)-cells and N-terminal sequencing of the final product

Purity

≥97%, as determined by SDS-PAGE(under reducing and non-reducing conditions) and visualized by Coomassie staining

Endotoxin level

< 1000 EU/mg, as determined by LAL gel clot test according to Ph.Eur.typically < 100 EU/mg

Residual host-cell DNA

≤200 ng/mg, as determined by a fluorimetric assay